Toward evidence-based severity assessment in mouse models with repeated seizures: I. Electrical kindling

ObjectiveEthical decisions about an allowance for animal experiments need to be based on scientifically sound information the burden and distress associated with experimental procedure models. Thereby, species differences considered recommendations regarding evidence-based severity assessment refinement measures.MethodsA comprehensive analysis of behavioral patterns corticosterone or its metabolites in serum feces was completed kindled mice. The impact kindling via two different stimulation sites amygdala hippocampus determined. Data were compared those from naive electrode-implanted groups.ResultsAmygdala mice exhibited comparable increased activity open field, reduced anxiety-associated behavior elevated-plus maze, anhedonia-associated saccharin preference test. In addition, repeated caused a reduction burrowing increase active social interaction. Levels not altered feces, respectively. A comparison mouse data findings rats confirmed pronounced process.SignificanceTaken together suggest classification paradigms as moderate regardless site. outcome provides valuable guidance selection studies exploring comorbidities. this context, it is emphasized that seem well suited link between ictal events network alterations one hand, hyperactivity other hand. Moreover, underlying pathophysiological mechanisms therapeutic interventions these can studied providing clinical management respective psychiatric comorbidities patients.